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Cells were washed in PBS, resuspended in Thiazole Orange (BD Retic-Count, BD Bioscience) containing 4 M Hoechst 33342 (Invitrogen) for parasite RNA and DNA detection respectively ( 2 , 5 mM glucose, 32 mM HEPES pH7.4, 5 mM KCl, 1 mM MgSO4, 125 mM NaCl), resuspended in 0.3 M of intracellular calcium dye x-Rhod-1 AM (Invitrogen) and incubated in the dark at 37C for 30 min

RF achieved a high AUC of 0.998 0.002 in 5-CV, while recall obtained with 0.992 0.007, respectively

Cavallo I, Sivori F, Mastrofrancesco A, Abril E, Pontone M, Di Domenico EG

The resultant elevation of oxidative radicals was found to be significantly reduced in cells further exposed to 250 M Sp (Fig

Brewer, Redox-Dependent Regulation of Sulfur Metabolism in Biomolecules: Implications for Cardiovascular Health