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It is also hypothesized that this leads to less glutamate available to produce aspartate, another excitatory neurotransmitter in the brain (Yudkoff et al., 2008)

Tumor necrosis factor alpha (TNF-) activates NF-kB through well-defined kinase pathways

To mitigate ethanol accumulation, feeding solutions were added at rates matching the glucose consumption rate, effectively maintaining ethanol levels below 15 g/L while supporting both cell growth and glutathione production

Nrf2 staining and translocation For visualization and quantification of endogenous Nrf2 translocation into the nucleus, glass 96-well plates were coated with fibronectin (1 g/ml) (Sigma) and poly-l-ornithine (40 g/ml) over night