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Briefly, 4-m sections of formalin-fixed and paraffin-embedded tissues were deparaffinized using Bond Dewax Solution (Leica Biosystems Newcastle Ltd.) and antigen retrieval was achieved by incubation with Bond Epitope Retrieval Solution 1 (Leica Biosystems Newcastle Ltd.) for 20 minutes at 98

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We assumed that a physician visit to explain to patients the benefits of oral therapy and instructions for use would take place on the day they came in to receive their intramuscular injection, and so this visit was not added to the conversion cost

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