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Detection of protein carbonylation Levels of carbonylated proteins were determined with the OxyBlot protein oxidation detection kit following the manufacturers protocol (Millipore, Billerica, MA, USA)

Our protocols include sample handling under mild, reducing conditions and immediate thiol blocking using alkylating reagents (e.g., NEM) to prevent artificial oxidation

No significant hepatotoxic, nephrotoxic, or immunosuppressive effects have been reported in preclinical literature at research concentrations [5,6]

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