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This can include over-the-counter medications like NSAIDs, antihistamines, and sleep aids, as well as supplements like calcium carbonate. When to talk to a doctor about bloating If you have persistent, troublesome bloating you should bring it up with a healthcare professional, especially if you also have stomach pain, diarrhea, vomiting, bad heartburn, or dark/bloody stools

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2.10 Immunofluorescence assay Cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.5% Triton X-100 for 20 minutes, and blocked with 10% goat serum for 30 minutes