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Vitamin B12 (cobalamin) is a water-soluble vitamin crucial for various physiological processes in the human body

About the author: Mary Genevieve Carty, MS, MHEd holds Masters degrees in Complementary and Integrative Health as well as Higher Education and is currently a doctoral student in Health Science at George Washington Universitys College of Medicine and Health Science

For whole islet staining, purified islets were fixed in 4% paraformaldehyde for 20 min and permeabilized with 0.3% Triton-X100 for 3 h at room temperature

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This is due to the adjacent positively charged nitrogen, which electrostatically stabilizes the carbanion formed upon proton dissociation