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For a 1 mg (1,000 mcg) target dose: Volume to Draw = Desired DoseConcentration = 1 mg5 mg/mL = 0.2 mL Convert to syringe units

An insulin syringe is an all-in-one unit where the needle is permanently attached to the syringe barrel

Dosing calculations translate directly to therapeutic exposure

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Western blots Adult retinal tissue pooled from 1-3 fish (tissue from 2-6 eyes) were homogenized in Pierce RIPA Buffer (ThermoFisher) lysis reagent (1 L/mg tissue) supplemented with 1X Halt Protease Inhibitor Cocktail (ThermoFisher) using a sterile microcentrifuge pestle and cordless pestle motor (VWR)