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Were trying to make our knowledge of metabolism more precise, Birsoy says

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The lowest standard concentration is the minimized range for reliable detection results

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E GSH in the mitochondrial matrix, the IMS and the cytosol recovers with similar kinetics To investigate the interplay of the glutathione pools of the cytosol, the IMS and the matrix, we first adapted the Grx1roGFP2 sensor for measurements in the mitochondrial compartments