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First, it can impair the transport of long-chain fatty acids into the mitochondrial matrix, with subsequent decrease in -oxidation, acetyl-CoA and ATP production

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[DOI] [PMC free article] [PubMed] [Google Scholar] 226.Davis M.E., Brewster M.E

The membrane was washed once with Tris-buffered saline with 0.1% Tween 20 (TBST) and incubated with a streptavidinalkaline phosphatase conjugate (KPL, medac, 1:2,000 in 1% BSA (Fraction V, Roche) in TBST) for 2 h at room temperature