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the gel purified digested His6-AV-Ubiquitin- semapeptide (nucleotide sequence) encoding gene PCR product was ligated into Ndel and Xhol sites of pET24 plasmid vector using Quick ligation kit (NEB)

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Plant Cell 11: 12771292 Dong Y, Silbermann M, Speiser A, Forieri I, Linster E, Poschet G, Allboje Samami A, Wanatabe M, Sticht C, Teleman AA, et al

Amylin is a naturally occurring peptide co-secreted with insulin by the pancreas, playing a critical role in regulating postprandial (after meal) blood glucose levels, slowing gastric emptying, and promoting a feeling of fullness

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