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Kang, E

This allows the water to remain safe for multiple uses over an extended period, often up to 30 days after the vial is first opened, provided proper storage and handling protocols are followed

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The test involves deliberately inoculating the preserved product with defined concentrations of five test microorganisms (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, and Aspergillus brasiliensis), then monitoring the microbial count over 28 days