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The paraffin-embedded colon samples were sliced into 7 m sections using a microtome (RM2145, Leica Microsystems K.K, Tokyo, Japan) and then mounted on glass slides (Matsunami-glass, Osaka, Japan)

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the delta values calculated from the initial and final values collected during the HFD period of 16 weeks were significantly higher in HFD group

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FIGURE 5 GSK3 negatively regulates CBS activity The transcription factor NF-E2-related factor 2 (Nrf2) encodes a protein which has a short half-time of only 30 min and which stability is controlled through the regulation of its turnover by the ubiquitin-proteasome system