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Data suggests that this could involve modulation of urea cycle enzymes in the liver and changes in messenger RNA expression

112 More recently, it was suggested that methotrexate causes ferroptosis through activation of autophagy, leading to increased degradation of ferritin and liberation of ferrous iron (ferritinophagy), which promotes LPO and liver injury

Western blot analysis further confirmed that H2O2 treatment led to increased levels of senescence-associated proteins p53, Ser15-phospho-p53, and p21

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