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Starting from RNA extracted with the Abbott m 2000 sp system, retrotranscription reactions were performed in a 30 l volume containing 7.5 l eluted RNA, 0.25 mM dNTPs, 150 U Moloney murine leukaemia virus reverse transcriptase and 6 l 5 RT buffer (Promega), 30 U RNasin RNase inhibitor (Promega), and 0.75 g random hexadeoxynucleotides (Roche) in order to retrotranscribe the whole HCV genome preventing any bias during the reaction

Any substance(s) in this category that produces intoxicating effects are banned for use for 48 hours prior to taking a polygraph examination with the WNBF

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