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Its possible that tirzepatide is slightly more effective because its a dual receptor agonist

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Efficacy and tolerability of sustained-release tramadol in the treatment of symptomatic osteoarthritis of the hip or knee: a multicenter randomized, double-blind, placebo-controlled study

After three washes with free-serum media, cells were collected, counted, and analyzed for their fluorescence intensity using a fluorescence microplate reader (Thermo Fisher Scientific, Waltham, MA, USA) with its excitation and emission wavelengths at 488 nm and 525 nm [29, 30]