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Stain-free gels were then wet-transferred onto polyvinylidene difluoride (PVDF, Millipore) membranes at 300 mV constantly for 1 h, and equal transfer was routinely confirmed by Ponceau S staining

Cognitive Impairment Models: Dihexa has been tested in scopolamine-induced amnesia models and aged rat models, where it demonstrated restoration of cognitive performance on spatial memory tasks

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Direct interaction between Nrf2 and p21(Cip1/WAF1) upregulates the Nrf2-mediated antioxidant response