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Although the scoping review does not include a formal risk of bias assessment, a critical discussion of the methodological quality of the included studies was conducted

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Culture was scaled up into a 1 L flask and grown for a further 4 hours, and protein production induced with 0.5 mM IPTG for 4 hours

Appropriate reasons to discuss discontinuation include achieving your weight loss goals and wanting to attempt weight maintenance without medication, experiencing intolerable side effects that don't improve with dose adjustment or supportive management, financial constraints that make continued treatment unsustainable, planning pregnancy (semaglutide is not recommended during pregnancy and should be discontinued at least two months before conception), or developing contraindications to continued use