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The samples were negatively stained with 2% uranyl acetate before being placed on the surface of the grid for observation after the copper gate was activated by ultraviolet light

Metabolic precursors of NAD +, such as nicotinamide mononucleotide (NMN) and nicotinamide (NR), increase intracellular NAD + availability, thereby reducing meiotic defects, supporting telomere maintenance, and improving follicle development [118, 119]

Cellular viability Optimization of Sp concentration and its cytotoxicity parameters were established by treating of 1.5 10 5 HeLa or 1.2 10 5 SHSY-5Y cells with serial concentrations of the Sp (100 M1 mM) for 15 min or conducting a time-course (5 min48 h) using 250 M Sp, followed by MTT assay as per standard protocol (Thermo Fisher Scientific)