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Following GPx-mediated H 2 O 2 consumption, residual hydrogen peroxide undergoes Fenton-type redox reactions with Fe 2+ ions, generating Fe + species that coordinate with Tiron through catechol moieties to form a stable ferri-Tiron complex [Fe(C 6 H 4 Na 2 O 8 S 2 )] +

To our knowledge, this has been documented to date in only one report showing that patients with NAFLD had increased hepatic protein glutathionylation, which was correlated with steatohepatitis and liver fibrosis 40

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